VBLセミナー・講習会 (2008年度)


平成20年度 第4回VBLセミナー


14:00〜14:45 Andrey Lomov (VBL招へい外国人研究員)

  " Investigation of semiconductor porous layers

     by high resolution  x-ray diffractometry and reflectometry "

14:45〜15:30 Nikolai Sokolov (VBL招へい外国人研究員)

  "Cobalt nanoparticles on fluoride surfaces:

    MBE-growth, structure and magnetic properties"


平成20年度 第3回VBLセミナー



United Research Center
United Institute of Technology, Allahabad, 211010, India & Ex Radiation Biology Biology and Health Sciences Division
Bhabha Atomic Research Centre, Mumbai 400 085
Email: mishra_kaushala@rediffmail.com mishradrkp@gmail.com

Exposure of biological cells to high intensity but short electrical pulses ( a few kV/cm, ?s duration ) has become an active area of biomedical research in the recent years. Plasma membrane of bacterial, plant and mammalian cells become transiently permeable on application of external electric field above a characteristic threshold value permitting exchange of molecules between cell interior and the surrounding. This phenomenon, popularly called electroporation, has opened many new possibilities for cell manipulation. For example, electroporation allows gaining direct access into the cell cytoplasm, transfer of gene into a variety of cells yielding efficient transformation and help achieve cell fusion for hybridoma technology. In our laboratory extensive studies have been carried out on electroporation of mammalian cells that have provided invaluable data on nature, lifetime and resealing properties of electroporated cells. Presently, molecular mechanisms of electro-permeabilization of cell are lacking. Potential of drug loaded red cells for achieving effective cancer treatment by targeted delivery of drugs appear highly promising. Our in vitro studies on Ehrlich ascites
cells have shown that at non- toxic concentrations of doxorubicin, an antitumor drug, produced substantial killing when drug treated cells were submitted to electroporation due to enhanced entry of the drug. These results have allowed designing a strategy to overcome tumor drug resistance by electroporation technology. More recent results from our laboratory have shown that tumor cells were sensitized to γ radiation effects when combined with electroporation. A highlight of the developing new approaches for cancer treatment and other biomedical applications will be outlined.


平成20年度 第2回VBLセミナー


            Prof. Devadasan Velmurugan  (VBL invited researcher 1 Sept. 2007−29 Sept. 2007)

             Centre of Advanced Study in Crystallography and Biophysics,

                              University of Madras, Guindy Campus, INDIA

For a better match of results between the computer modelling and wet lab experiments, Induced Fit algorithm is used in docking programs.GLIDE program of the SCHOEDINGER USA , the commertial software and MOE were used in docking studies for HDAC inhibitors towards anti-cancer drug design, Pyrrolidine analogues for anti-diabetic properties, plant and marine products towards anti-inflammatory drug design. MD studies have also been undertaken for studies of PLA2-Vitamin E complex. Results based on the above studies will be presented.Three dimensional X-ray crystal structures of some of the ligand molecules used in the above studies were from our research group.

                             Dr. Amit Sharma  (VBL invited researcher 2 Sept. 2007−26 Sept. 2007)


                             NEW DELHI, INDIA

The group aims at understanding the structural principles that govern protein-based bio-molecular interactions in malaria parasite. Structure-function studies on several important malaria parasite proteins are currently under way. Our laboratory uses multi-disciplinary techniques within modern biology, including biochemistry, bioinformatics, cell biology, parasitology and protein crystallography, to unravel the mechanism of action of important parasite proteins. Over the past few years, we have made progress in understanding various parasite proteins involved in erythrocyte invasion. We have solved crystal structures of novel proteins from each of the above stages of the parasite life cycle. We will discuss results on several such studies involving unique malaria parasite proteins.

平成20年度 第1回VBLセミナー